TissueQuest

Fluorescence image analysis for cell and tissue markers, including single-cell quantification and co-expression assessment.

Overview

TissueQuest is a fluorescence image analysis software developed by TissueGnostics, designed for biological and clinical research applications. It supports the analysis of fluorescent markers across a range of sample types, including tissue sections, tissue microarrays (TMAs), confluent cells, and smears. The software is available both as part of the TissueGnostics platform and as a standalone application.

TissueQuest offers three core analysis modes: cell-based analysis covering nucleus, cytoplasm, and membrane compartments; stained area analysis; and small dot detection, such as for FISH applications. Context-based analysis is supported through manual definition of regions of interest (ROIs), and TMA detection and analysis can be performed on imported files.

Analysis Capabilities

Fluorescence image analysis across multiple sample types
Single cell analysis with nuclear segmentation
Cytoplasmic and membrane measurements
Total area measurements and dot detection
Assessment of co-expressed markers
18 measured metrics per marker
Pseudo IHC functionality

Workflow and Usability Features

Streamlined workflow for image acquisition and analysis
Contextual image analysis via manual ROI definition
TMA detection and analysis on imported files
Image and data comparison tools

TissueQuest is developed by TissueGnostics GmbH, based in Vienna, Austria. The company focuses on whole-slide imaging and image analysis solutions for biological and clinical research, with products aimed at enabling detailed examination of tissue structure, cellular interactions, and spatial cell landscapes.