NanoString nCounter Gene and miRNA Expression Analysis

ROSALIND is Bruker's recommended and preferred solution for best-in-class NanoString nCounter gene and miRNA expression data analysis. As NanoString's strategic partner for data analysis, ROSALIND integrates directly with nCounter instruments to deliver a streamlined, end-to-end analytical workflow — accepting both RAW RCC files and processed data exports. The platform is purpose-built to extract the deepest, most reliable insights from targeted gene panels, making it essential for researchers in drug discovery, grant-driven science, and translational research.

Designed for scientists at every skill level, ROSALIND requires no programming or bioinformatics expertise. From the lab bench through downstream analysis, collaboration, and pathway exploration, researchers retain full control of their data without relying on outdated or difficult-to-use free software tools.

Core Analytical Capabilities

• Custom Low Expressor Pruning: ROSALIND automates the identification and removal of low-expression probes using advanced algorithms, reducing background noise and preventing misleading results caused by random fluctuations in low-expressed genes. Gene pruning and normalization are performed in the correct sequence to ensure more reliable insights.
• Custom Normalization with geNORM: The platform integrates geNORM's advanced capabilities, enabling researchers to identify and select the most stable reference genes for precise normalization. Selected normalization probes can be exported for consistent application across multiple analyses, supporting reproducibility and data integrity.
• Cell Type Profiling: Advanced cell type profiling is included as part of the platform's enhanced nCounter analysis capabilities.
• Robust QC Tools: Comprehensive quality control tools are built in to ensure high-quality results at every stage of analysis.
• Multiple Pathway Databases with p-value Support: ROSALIND provides access to multiple pathway databases, enabling deeper biological interpretation compared to nSolver.

Rosalind Intelligence and Meta-Analysis

• AI-Powered Literature Reviews: Rosalind Intelligence harnesses generative AI grounded in biological truth via the Rosalind Knowledge Graph, producing rich literature reviews on target genes of interest in seconds.
• Public Repository Correlations: Researchers can explore correlations to all available nCounter data from public repositories, uncovering hidden links within their data that drive groundbreaking findings.
• Multi-Omics Meta-Analysis: ROSALIND enables integration of nCounter datasets with diverse multi-omics data layers, allowing researchers to compare datasets with precision or weave them into a broader multi-omics context. This integrative approach provides a holistic understanding of complex biological systems and serves as a data mining foundation for future insight harvesting.

Collaboration and Sharing

• Collaboration Spaces: A dynamic online environment connects team members across labs, sites, and continents, providing access to fully visualized datasets with complete pathway interpretation and analysis tools.
• Flexible Subscription Tiers: Academic, Professional, and Enterprise subscriptions enable team members to explore, re-analyze, add new cut-offs and comparisons, and set up new meta-analyses across datasets — going well beyond the basic sharing available in Starter subscriptions.
• Normalization Export for Team Alignment: Teams can share selected normalization probes to ensure uniform analysis standards across collaborative projects, supporting both synchronized normalization and advanced offline bioinformatics workflows.

Advantages Over nSolver

• Continuously updated with no maintenance required, ensuring access to the latest features at all times
• Correct sequencing of gene pruning and normalization for more reliable results
• Access to multiple pathway databases with p-value support for deeper analysis
• Real-time collaboration tools and shared libraries for seamless teamwork
• Robust QC and meta-analysis tools built into the platform

Expert Support

• A dedicated team of nCounter specialists provides in-depth knowledge and guidance that goes beyond basic support offerings
• PhD-level Customer Success team members combine technical acuity with deep scientific domain understanding to ensure analyses are both technically sound and strategically aligned with research goals

ROSALIND is available to pharma, biotech, and academic researchers through Enterprise, Professional, and Academic subscriptions, as well as specialized licenses for service providers, contract research organizations (CROs), and academic core labs. A 30-day free premium access trial is available prior to purchase.