FCS Express

FCS Express, developed by De Novo Software, is a flow cytometry data analysis software that takes raw cytometry data and produces formatted, presentation-ready results. It is designed for a range of users including individual academic and corporate R&D researchers, shared resource facilities, clinical diagnostic labs, clinical reference labs, and cell and gene therapy researchers with GxP requirements.

The software is built to resemble familiar Microsoft Office applications such as PowerPoint and Excel, with the intent of reducing the learning curve for new users. It is positioned as an alternative to other flow cytometry analysis tools, including FlowJo, particularly for users who need integrated reporting and analysis without relying on copy-and-paste workflows between applications.

Target User Groups and Editions

• Academic and Corporate R&D: Supports individual researchers and shared resource facilities in moving from raw cytometry data to clearly communicated results.
• Clinical and Diagnostic: Described as widely used among clinical diagnostic and clinical reference laboratories, with features suited to high-volume environments and complex multicolor analysis.
• Validation Ready Package: Assembled specifically for cell and gene therapy researchers who need to meet GxP requirements.

Key Features and Capabilities

• Familiar interface modeled on Microsoft Office applications, reducing the time needed to learn the software.
• Integrated spreadsheets that link directly to gates and data files, updating in real time as gates move or data changes, eliminating manual copy-and-paste steps.
• Support for complex multicolor analysis workflows.
• Ability to generate high-quality reports suitable for clinical clients and publication.
• Token, alert, and panel features that support iterative improvement of analysis protocols.
• Virtual component options for customers who want remote or tech-only flow cytometry access or closer interaction with patient results.
• Designed to scale for high-volume reference laboratory environments, with one user citing workflows covering over 350 cases per day across a company.

Integration with EasyPanel

• FCS Express integrates with EasyPanel to support quality control and validation of fluorochrome reagents.
• Compares actual spectral signatures of single-color controls, generated experimentally on the instrument and uploaded into FCS Express, against expected signatures.
• Accounts for autofluorescence spectral signatures from unstained controls imported into FCS Express to further optimize and QC panels.

Available Versions

• FCS Express RUO (Research Use Only) — current version 7.30.0018
• FCS Express IVD (In Vitro Diagnostic) — current version 7.30.0018
• Previous versions are also available for download.

FCS Express is available as a free trial and offers pricing tiers to accommodate different laboratory types. The software is developed and supported by De Novo Software, and user testimonials consistently highlight the responsiveness and quality of the technical support team as a notable aspect of the product offering.