De Novo Software

De Novo Software develops FCS Express™, a flow cytometry data analysis software designed to take researchers and clinical laboratory professionals from raw cytometry data to formatted, presentation-ready results. The software is used across academic research, corporate R&D, clinical diagnostic laboratories, and cell and gene therapy settings, and is described as the most widely used flow cytometry data analysis software among clinical labs.

FCS Express™ is built to resemble familiar Microsoft Office applications such as PowerPoint and Excel, with the intent of reducing the learning curve associated with flow cytometry software. The platform supports complex multicolor analysis, real-time linked spreadsheets, reporting, and panel quality control, and is positioned as an alternative to tools such as FlowJo for users seeking integrated workflows and publication-quality outputs.

Target User Segments

• Academic and Corporate R&D: Serves individual researchers and shared resource facilities, enabling users to move from raw cytometry data to clearly communicated results.
• Clinical and Diagnostic Labs: Designed for clinical diagnostic and clinical reference laboratories, with features supporting high-volume workflows. Labcorp, for example, uses FCS Express to manage over 350 cases per day across its operations.
• Cell and Gene Therapy Researchers: Offers a Validation Ready Package assembled to help researchers meet GxP requirements and compliance needs.

Core Features and Capabilities

• Familiar interface: Designed to look and function like Microsoft Office programs, reducing onboarding time for new users.
• Integrated spreadsheets: Spreadsheets can be embedded directly within the analysis environment and linked to gates and data files, updating in real time as gates move or data changes, eliminating the need to copy and paste results into separate applications.
• Tokens, alerts, and panels: Supports advanced analysis features including tokens, alerts, and panel configurations that allow users to improve analysis quality and consistency.
• High-quality reporting: Produces formatted reports suitable for clients, publications, and presentations.
• FlowJo migration support: Provides resources and guidance for users switching from FlowJo to FCS Express™.

Integration with EasyPanel

• FCS Express™ integrates with EasyPanel, a panel design and inventory management tool for flow cytometry.
• The integration allows users to automatically QC and validate the quality of fluorochrome reagents by comparing actual spectral signatures of single-color controls — generated experimentally on the instrument and uploaded into FCS Express™ — against expected signatures.
• Users can further optimize and QC panels by incorporating the autofluorescence spectral signatures of unstained controls imported into FCS Express™.

Notable Customers and Use Cases

• Used at City of Hope, with researchers citing responsive technical support.
• Adopted by Laboratory Corporation of America (Labcorp) for companywide clinical flow cytometry workflows, selected for its user-friendly interface, complex multicolor analysis support, high-quality client reporting, and scalability for high-volume reference laboratory environments.
• Used at Shands Teaching Hospital at the University of Florida, where clinical flow cytometry laboratory staff noted the software's features including tokens, alerts, and panels as tools for improving analysis quality.

De Novo Software offers a free trial of FCS Express™ and provides tiered pricing. The company is noted by multiple customers for the quality and responsiveness of its technical support team.