Byos

Byos, developed by Protein Metrics, is a desktop software platform designed for comprehensive biotherapeutic protein characterization using mass spectrometry data. Built for scientists across the full product development lifecycle, Byos is a vendor-neutral, instrument-agnostic application that processes raw mass spectrometry data from any spectrometer and delivers analysis, inspection, and reports in minutes. It is suited for both expert and non-expert users working on complex biopharmaceutical candidates.

Byos automates the mass spectrometry analysis pipeline end-to-end, offering templated reporting and unique algorithms that minimize ambiguity, ensure reproducibility, and eliminate lab-to-lab variability. Its clean, intuitive interface provides access to all tools needed for rapid, confident protein analysis, including the ability to quantify species down to trace amounts. For organizations requiring enterprise-level capabilities, Byos can be upgraded to the Byosphere platform for advanced analysis, reporting, and collaboration.

Intact, Sub-Unit, and Native Analysis

• Automatic MS/MS spectral annotation and MS-level deconvolution
• Ligand binding and protein-protein interaction analysis
• Protein and subunit identification
• Glyco-profiling
• ADC and DAR analysis
• Charge variants by iCIEF-MS and CE-MS

Peptide-Level Analysis

• Integrated workflow covering sample preparation, peptide identification, data processing, and reporting
• Hotspot analysis and peptide mapping
• Post-translational modification (PTM) analysis
• Host cell protein (HCP) analysis
• Sequence variant analysis
• Epitope mapping

Multi-Protein Quantitation

• Label-free, relative quantification of complex protein mixtures
• Proteome profiling and full protein characterization
• Rapid sample prep and MS quality check
• Automated analysis for faster cell line selection and curated HCP quantitation
• Templated dashboards that simplify complex LC-MS/MS data for non-expert users in real time

Chromatogram Analysis

• Peak annotation and chromatogram alignment
• Peak and trace comparison
• Released glycan analysis
• MAM workflows and CE-UV, SEC-UV, LC-UV, and other chromatographic workflows
• Import of 2D trace data and automated report generation

Higher Order Structure

• HDX and FPOP workflows
• Epitope mapping, paratope mapping, and other higher order structure analyses
• Representation in .pdb format

Additional Workflow Capabilities

• Oligonucleotide workflows including impurities, clips, and sequence confirmation for known oligos
• De novo sequencing with hands-free, automated sequencing of purified monoclonal antibodies
• Intact Reconstruction for correlating deconvoluted intact data with peptide mapping data, PTM quantification, degradation or conjugation analyses, and acquisition parameter optimization
• MOBILion HRIM workflows including released glycan, intact, and peptide mapping for the Mobie platform
• icIEF-MS workflows with representation on a pI axis and deconvolution

Byos is a one-click workflow platform that supports the entire biopharmaceutical development process. Each workflow automatically populates reports and summaries from templates, ensuring consistency across teams and sites. Educational resources including videos, guides, webinars, and a knowledge base are available to help users maximize the platform's capabilities.