Yamaha Cell Handler

Overview

The Yamaha Cell Handler™ revolutionizes cell isolation and imaging by offering a high-speed automated system ideal for clonal selection, single-cell sequencing, and isolating individual stem cells as small as 10µm. This system also efficiently handles spheroids, organoids, or PDX samples up to 180µm.

The system uses a built-in microscope to image and identify cells, employing custom precision tips for gentle aspiration and dispensing.

Features

• Operates in manual, automatic, or machine learning modes for cell and spheroid selection.
• Supports up to three color fluorescence imaging and bright field imaging.
• Capable of handling objects from 10µm to 2.0mm.
• Completes a 384-well plate in under 30 minutes.
• Images serve as permanent records and provide metrics like area and roundness.

Operation Modes

Manual mode allows users to select cells via a touchscreen. Automatic mode uses user-defined thresholds, and machine learning mode utilizes algorithms to define selection criteria.

Precision and Quality Control

High-resolution images verify single cell transfers, ensuring accuracy. Users can mark wells if multiple cells are transferred, preventing their use in further experiments.

Technical Specifications

• Includes up to three excitation and emission filters.
• Environmental controls with HEPA filters.
• Compatible with automation, with consultation needed.

The Yamaha Precision Chamber ensures a high success rate for single spheroid selections, preventing crowding of spheroids. Precision Tips, designed for reuse, further enhance accuracy in transfers, while developments are underway for larger reusable tips.