IsoSpeak

IsoSpeak is a sophisticated data visualization software designed for the IsoLight system, enabling users to gain insights into immune cell functionality and communication. It provides automated on-site analysis and advanced functional single-cell mapping, allowing researchers to visualize complex data with intuitive and meaningful visualizations.

With IsoSpeak, users can connect the dots and obtain answers swiftly through detailed informatics and statistics on cellular potency and durability. The software also facilitates efficient project organization and sample annotation, streamlining immunotherapy workflows.

Push-Button Informatics

IsoSpeak offers push-button informatics to accelerate workflows, featuring:

• 3D visualizations for donor group cell mapping, distinguishing responders from non-responders.
• Polyfunctionality mapping to display data points based on cytokine secretion levels.
• Dominant Functional Group mapping for color-coded visualization of cytokine profiles.

The software employs t-SNE functional graphs to differentiate cellular fitness by cytokine subsets, revealing functional sources of cellular biomarkers of response. It visualizes stratification differences in responders versus non-responders, as demonstrated in numerous precedent datasets.

Unique Visualization Options

IsoSpeak provides unique visualization options to highlight the impact of polyfunctionality, including:

• Automated, parallelized image processing for data extraction and quality control.
• Sample annotation and project organization for immunotherapy workflows.
• Comprehensive informatics and statistics for comparison and correlation across experiments.

The Polyfunctional Strength Index (PSI) aggregates multi-dimensional cytokine secretions into a single index, color-coded to show contributions from different cytokine categories. Additionally, Polyfunctional PCA graphs stratify donor/patient responses by cellular cytokine signatures, highlighting dominant subgroups driving responses.

IsoSpeak's user-friendly interface and advanced automation enable users to visualize and utilize data from direct functional cytokine profiling of single cells efficiently, ensuring mastery of data quickly.