Impedance Module

The Impedance Module offers a comprehensive solution for tracking various cellular activities such as proliferation, viability, and morphology without the need for labels, and in real time, across every well of a multiwell plate. Designed for seamless integration with Maestro Pro, Maestro Edge, Maestro Z, and Maestro ZHT systems, this module simplifies the process of conducting impedance-based assays efficiently.

Using AxIS Z software, the Impedance Module allows for straightforward setup, execution, and analysis of experiments. It facilitates easy control over experimental settings, provides real-time cellular profiling, and enables in-depth analysis of cytolysis and barrier functions. The module supports effortless assay setup with features like automatic data acquisition and pre-configured analysis settings while still allowing access to advanced settings as needed.

Features

• Real-time visualization with continuous data display across the plate, enabling comparisons between treatment groups.
• Remote monitoring capabilities via the Maestro Z App to ensure experiment continuity.
• Data analysis tools that include plotting cytolysis curves, dose-response analysis, and evaluating cell membrane permeability with AxIS Z's Barrier Index.
• Generate publication-ready figures and data tables using the interactive Lab Notebook, with export options to Microsoft Excel.

Impedance assays provide label-free and kinetic data beneficial for understanding cellular interactions and drug responses in detail. Cells are cultured on CytoView-Z plates with electrodes, where changes in impedance quantify the cell presence on the electrode, indicating physiological changes like attachment, spreading, and cell-cell interaction.

Applications

• Assessment of monoclonal antibodies in enhancing cellular cytotoxicity.
• Real-time tracking of antiviral effects through cytopathic changes.
• Evaluation of cell barrier integrity using TEER assays.
• Modeling solid tumor biology to study immunotherapies and anticancer agents.
• Tracking cell migration, invasion, and growth, and investigating GPCR cell signaling.

Overall, the Impedance Module is a versatile and advanced platform for conducting a wide range of cellular assays, offering actionable insights into cell health and behavior, especially in contexts like cancer studies and drug efficacy testing.