Impedance Module

The Impedance Module is designed for use with Maestro instruments to enable real-time, label-free analysis of cell activities including proliferation, viability, morphology, and immune-mediated effects. It employs impedance-based assays to provide continuous data, highlighting cell interactions and drug responses without the need for multiple endpoint assays.

Key features include the AxIS Z software that simplifies the setup, execution, and analysis of experiments, offering both beginners and experienced users the ability to manage impedance assays effectively.

Assay Setup

Setting up assays is streamlined with automatic data acquisition and pre-configured analysis settings, while advanced options are available for specific experimental needs. The color-coded plate map allows real-time tracking of experiments and control types, enhancing analysis automation.

Real-time Visualization

Continuous impedance data is displayed for all treatment groups, allowing for immediate comparison and analysis. Important events can be annotated with time-stamped notes to aid later data correction or normalization. Remote monitoring of experiments is possible with the Maestro Z App.

Data Analysis Tools

• Create cytolysis curves and determine KT50 values.
• Perform dose response analysis with automatic computation of EC50/IC50.
• Investigate cell membrane permeability using the Barrier Index.
• Export publication-ready figures directly to Excel via the interactive Lab Notebook.

Cells are cultured on electrodes in Axion’s CytoView-Z plates. Impedance measurements, conveyed in ohms, quantify cell presence by gauging the electrical signal passage through the cell-electrode interface. Increased impedance indicates cell attachment and morphological changes, while decreased impedance signals cell death or detachment.

The Impedance Module supports various applications, such as evaluating monoclonal antibodies, monitoring antiviral effects and cell barrier integrity, modeling solid tumor biology, and assessing cellular signaling dynamics. It is ideal for tracking cell migration, invasion, and viability, and understanding immune cell-mediated cytotoxicity.