Fluorescence Module

The Fluorescence Module enhances live-cell imaging capabilities, integrating with the Omni™ and Lux™ systems. It supports fluorescent assays utilizing green and red channels to measure parameters such as fluorescence confluency, intensity, and object count directly within incubator environments. This functionality is pivotal for analyzing cell cultures in real-time.

Key Features:

• Versatile fluorescence assays: Enable real-time analysis of complex biological processes by adding fluorescence capabilities to your existing systems.
• Efficient data visualization: View and navigate multiple image snapshots efficiently using the FL map view.
• Simple fluorescence acquisition: Automate and expedite image capture, keeping cells undisturbed in the incubator.
• Automated analysis: Instantly access analyzable results, facilitating the generation of publication-ready data.

Study Cellular Processes with Fluorescent Live-Cell Assays

This module permits the observation of kinetic cellular processes over time, allowing quantification of fluorescent markers to investigate processes such as proliferation, cytotoxicity, transfection, and 3D cell organization.

Kinetic Cell Killing Assays

By using GFP-labeled cancer cells, immune cell-mediated killing by CAR T cells can be quantified over time, displaying a dose-dependent response through changes in fluorescence intensity.

Measuring 3D Culture Fluorescence

Real-time imaging facilitates the tracking of spheroidal formation in GFP-labeled cells, measuring changes in size and fluorescence intensity as they develop.

Quantifiable Metrics:

• Confluency (%)
• Intensity
• Object count
• Object area (µm²)
• Object intensity
• Average object intensity

Applications: This module is ideal for monitoring cellular growth, assessing cell health, and understanding behavior. It also aids in evaluating cell viability and determining cytotoxic effects of compounds through acute and chronic exposure experiments, alongside tracking transfection efficiency and expression levels in cultured cells.