Exact FL

The Exact FL is designed for the precise and automated counting of cells, an essential process in various experimental, clinical, and diagnostic workflows. Utilizing advanced optics and cutting-edge image analysis software, it provides unparalleled detail for distinguishing cells from debris. It can accurately detect individual cells in clusters and measure the size of organoids.

Key advantages of the Exact FL include:

• Speed and Consistency: By using machine-learning software, this platform reduces user variability and enhances reproducibility, allowing scientists to concentrate on their experiments without the tedium of manual counting.
• Fluorescence Capabilities: Equipped with red and green fluorescence channels, it can count specific cell populations, in addition to providing cell number and viability data through brightfield imaging.
• Ease of Use: The device is user-friendly, requiring no calibration, and it can be quickly set up in any laboratory due to its maintenance-free design.

The Exact FL efficiently combines brightfield and fluorescence-based methods with sophisticated CMOS-based optics and AI-driven analysis to provide dependable and reproducible results for routine cell counting. It can seamlessly integrate with existing workflows, offering compatibility with various reusable and disposable counting slides and supports analysis through advanced machine-learning algorithms.

Software/Algorithms

The platform is versatile, supporting both brightfield and fluorescence measurements. It accurately gauges cell concentration, size, and viability, making it suitable for tasks such as cell seeding, protocol standardization, and assessing cell growth.

Furthermore, the Exact FL can assess cell viability using either green/red fluorescence channels (AO/PI) or a brightfield channel (Trypan Blue). It also works with any transparent cell counting chamber and provides optimal fluorescence results if the device stage is covered to minimize environmental light.

The system requires a Windows 10 or later desktop or laptop with USB3 and internet connectivity for accessing the Axion Portal for image analysis and storage.

Maintenance of the Exact FL is straightforward. It can be cleaned with lint-free wipes and 70% ethanol or isopropyl alcohol, ensuring long-term usability without demanding cleaning methods like autoclaving.

The Exact FL supports applications like measuring cell concentration, size, and viability in single cell or organoid cultures, enhancing standardization and efficiency in scientific research.