ClearScope

The ClearScope light sheet theta microscope is designed for high-resolution imaging of cleared biological specimens across various sizes, providing subcellular resolution. Developed collaboratively by Dr. Raju Tomer from Columbia University and MBF Bioscience, this system effectively addresses the challenges of imaging cleared organs and brain tissues.

The instrument is equipped to handle specimens that surpass the capacities of conventional light sheet imaging, from small samples to large tissues like primate brains. It supports rapid 3D imaging while minimizing photobleaching, thanks to its flexible configurations compatible with multiple objective lenses and up to seven wavelengths.

Key Features and Benefits

• Adaptable hardware design with customizable components to suit diverse research needs.
• Suitable for a wide range of clearing methods using Intelligent Refractive Index Compensation (IRIC).
• Efficient data processing with advanced GPU-acceleration, seamless stitching, and compression.
• High numerical aperture and long working distance enable better optical sectioning with uniform illumination.

The ClearScope software, BrightSlice, integrates acquisition, processing, and visualization in a single platform, supporting teravoxel-scale imaging efficiently. The software includes features like real-time microscope control, multi-channel imaging, and advanced processing capabilities, optimized for high-performance computing environments.

Overall, ClearScope stands out as a comprehensive solution for advanced light sheet imaging, allowing users to fully control imaging parameters or engage with user-friendly automated modes for streamlined operations.