Spotxel Microarray

Spotxel® Microarray is a user-friendly and intuitive software solution for comprehensive microarray image and data analysis. Designed to support protein microarrays, antibody microarrays, peptide microarrays, and gene microarrays, it provides researchers with a robust set of tools to streamline image handling, automate processing, and enhance data quality. The software is compatible with both Windows and macOS platforms, ensuring seamless integration into existing laboratory workflows.

Spotxel® Microarray has been used in peer-reviewed research published in leading journals including Science Advances, Nature Communications, Cell Host & Microbe, Journal of Experimental Medicine, and Scientific Reports, covering areas such as HIV-1 neutralizing antibodies, SARS-CoV-2 immunity, hepatitis E virus neutralization, bat antibody diversity, and autoantigen identification in rheumatoid arthritis.

Key Features

• Microarray Image Handling: Provides robust tools for efficiently loading and viewing microarray images, available immediately upon installation at no cost.
• GAL Array Editor Module: Offers a comprehensive and user-friendly interface for editing array design and layout files, accessible for free upon software installation.
• Premium Features: Enables advanced functionalities including data quantification, automated array alignment, high-throughput batch processing of multiple images, replicate processing, data filtering, and data normalization, available with a licensed version.

Key Benefits and Workflow Efficiency

• The microarray image analysis process is simplified into four steps: Load image(s), Load or create array, Align array, and Quantify.
• The array alignment function works well on microarray images with standard spot layouts, with tunable parameters available for custom layouts.
• Batch processing dramatically reduces manual processing time — approximately 160 microarray images that would take 3 weeks to process manually can be completed in just 2–3 hours.
• Supports multiple screens, allowing users to display the graphical canvas with the array image on one screen and the data table on another for efficient analysis.

Data Quality and Spot Detection

• Advanced noise processing technology with background and foreground correction ensures high-quality results.
• Data filtering, normalization, and replicate processing tools are included to maintain data integrity.
• Powerful detection algorithms ensure accurate spot identification even when spot shapes and positions deviate from specifications.

Compatibility and File Format Support

• Compatible with TIFF images from a wide variety of scanners and cameras, including those not specifically designed for microarrays.
• Supports 8-bit, 16-bit, and 24-bit grayscale TIFF images, with or without resolution information, in single-page or multi-page formats.
• Uses the widely adopted GAL format for array layouts.
• Supports both rectangular and hexagonal (orange packing) spot layouts.
• Compatible with arrays stored as PepSlide Designer files (PSF) (*.psf).
• Exports results in CSV and GPR formats for easy downstream analysis.

Licensing and Trial Options

• Free image handling and array editor tools are available immediately upon installation.
• A 14-day free trial provides access to all premium functions, available for download on Windows and macOS.
• Flexible licensing options are available, including 3-month, 1-year, and time-unlimited models.