Pulse

Pulse is a suite of contractile tissue software solutions developed by Curi Bio, designed to support quantitative analysis of stem cell-derived and primary cardiomyocytes, as well as 3D engineered tissues. The platform is aimed at researchers conducting drug discovery, cardiotoxicity screening, and functional tissue studies who require high-throughput video-based contractility analysis.

The Pulse suite includes two primary software modules — Pulse 2D and Pulse 3D — each targeting different experimental formats, from standard well-plate 2D cell cultures to more complex 3D engineered tissue models.

Pulse 2D — High-Throughput Contractility Video Analysis

• Supports calcium measurement using image intensity analysis
• Measures contractility and beat activity using correlation analysis and optical flow methods
• Allows post-analysis comparison of results across experiments
• Available as desktop software for both Windows and MacOS
• Supports batch upload of videos for high-throughput analysis across 96-well and 384-well plate experiments

Pulse 3D — High-Throughput Analysis of 3D Engineered Tissues

• Designed for analysis of 3D engineered tissue constructs
• Measures force frequency relationships
• Tracks rate of contraction (systole) and rate of relaxation (diastole)
• Captures time to maximum twitch (peak)
• Measures maximal twitch amplitude
• Calculates full width at half maximum (FWHM) of contractile events

Related Platform — Nautilai Optical Mapping

• Provides optical mapping of voltage and calcium for electrophysiology screening
• Supports high-throughput analysis of calcium transients on standard SBS-compliant plates
• Compatible with both 2D and 3D cell formats
• Applicable to calcium flux studies, cardiomyocyte screening, cardiotoxicity assessment, potassium assays, and ion channel research

Pulse is part of Curi Bio's broader data analytics platform offering, which complements their biosystem hardware platforms including the Mantarray and Nautilai systems. The desktop deployment model supports integration into existing laboratory workflows for plate-based contractility studies.