CopyCount

CopyCount™ is a qPCR analysis software developed by DNA Software that determines the absolute number of DNA or RNA copies in every well of a qPCR plate. It is designed for researchers who need absolute quantification — such as measuring viral load, bacterial load, or standardizing reference stocks — without the cost and complexity of Digital PCR instrumentation or traditional standard curve methods.

The software works by analyzing the shape of qPCR fluorescence curves to deduce the absolute copy number at cycle zero. This approach eliminates the need for standard curves, sample dilution, or excessive replicates, and is compatible with existing qPCR instruments rather than requiring new equipment purchases.

Core Capabilities

• Determines absolute DNA/RNA copy number directly from individual qPCR fluorescence curves, without a standard curve
• Reports copy number at cycle zero, removing the need to interpret Ct or Cq values that vary across assays, machines, and plates
• Delivers copy number results reported to be 3–4 times more precise than the Ct method
• Provides absolute quantification within 5% error using existing lab equipment and methods
• Supports applications including viral load determination, bacterial load measurement, and reference stock standardization

Advantages Over Standard Curve and Digital PCR Methods

• Eliminates the preparation and running of quantification standards, reducing reagent use and freeing up plate wells for unknown samples
• Removes two common sources of user error: standard quantification and standard curve generation
• Offers higher sample throughput than Digital PCR while using existing qPCR instrumentation
• Reduces cost per well by replacing control wells with additional sample wells
• Functions as a cost-effective alternative to Digital PCR without requiring new capital equipment

Supported Instrument Formats

• Abbott m2000rt
• ABI 7500/7900
• Bio-Rad: CFX and iCycler
• Fluidigm
• Life Technologies: ViiA 7, Step One, OpenArray 12K Flex
• Qiagen: Rotor-Gene
• Roche: LC480 and Lightcycler Series
• StrataGene MX
• General Column-Layout CSV
• General Row-Layout CSV

Data Input Requirements

• Accepts raw qPCR fluorescence data uploaded as text-based CSV or TSV files, or Excel XLS/XLSX formats
• Does not currently support direct binary formats such as SDS and EDS files
• Automatically detects data from the listed instrument formats; additional formats can be requested from DNA Software

CopyCount™ includes a two-step assay calibration procedure for TaqMan assays and is supported by a validated method whitepaper. A quick start guide is available, and demo scheduling is offered for organizations evaluating the software.